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Transcriptome Analysis Reveals Critical Genes Involved in the Response of Stropharia rugosoannulata to High Temperature and Drought Stress

Summary

This study examined how a type of edible mushroom called Stropharia rugosoannulata responds to high heat and dry conditions by analyzing which genes become active. Researchers found that under stress, the mushroom activates specific genes that help it survive, particularly through a cellular communication pathway called MAPK. They identified 15 key genes that could be useful for breeding mushroom varieties that better withstand harsh growing conditions, which could improve mushroom farming efficiency.

Background

Stropharia rugosoannulata is an important edible and medicinal mushroom with high nutritional value. High temperature and drought stress negatively impact mycelium growth, fruiting body development, and yield. Understanding the molecular mechanisms of stress response is critical for developing stress-tolerant varieties.

Objective

To analyze gene expression differences in S. rugosoannulata under high temperature and drought stress using transcriptomics. To identify and characterize critical candidate genes involved in stress response mechanisms and verify their expression patterns.

Results

A total of 74,571 transcripts and 16,233 unigenes were identified. Under high-temperature stress, 798, 851, and 1,484 DEGs were identified at 1, 2, and 3 days respectively. Under drought stress, 421, 1,072, and 2,880 DEGs were identified at 3, 6, and 9 days respectively. The MAPK signaling pathway (ko04011) was significantly enriched in all treatment groups, and 15 candidate genes were identified.

Conclusion

This is the first comprehensive transcriptome analysis of S. rugosoannulata under high temperature and drought stress, identifying 15 candidate genes potentially involved in stress response. The findings provide valuable gene resources and molecular insights for breeding stress-resistant varieties and improving cultivation practices.
  • Published in:Current Issues in Molecular Biology,
  • Study Type:Experimental Study,
  • Source: 10.3390/cimb47100835, PMID: 41150782
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