Transcriptome Analysis Explored the Differential Genes’ Expression During the Development of the Stropharia rugosoannulata Fruiting Body

Author: mycolabadmin
6/29/2022
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Summary

Researchers studied how S. rugosoannulata mushrooms grow and develop by analyzing which genes are active at different stages of fruit body formation. They found that the mushroom’s development relies heavily on glucose and amino acid metabolism, with special genetic processes called alternative splicing playing key roles in maturation. This is the first comprehensive genetic study of this edible mushroom’s development, providing valuable information for improving cultivation techniques and mushroom quality.

Background

Stropharia rugosoannulata is an edible fungus with high medicinal and nutritional values that is environmentally friendly and recommended by the FAO as a food source. Despite its importance, the molecular mechanism regulating fruiting body development remains poorly understood. This study addresses the need to identify genes and regulatory pathways controlling fruiting body development through transcriptome analysis.

Objective

This study aimed to analyze differentially expressed genes (DEGs) and alternative splicing events across four developmental stages of S. rugosoannulata fruiting bodies: primordia stage, young mushroom stage, picking stage, and opening umbrella stage. The goal was to clarify the molecular mechanisms underlying fruiting body development and maturation.

Results

A total of 3,112 DEGs were identified across three pairwise comparisons, with the largest number (2,374) between primordia and young mushroom stages. GO analysis showed 21 significantly enriched terms in the primordia vs. young mushroom comparison, primarily involving transmembrane transport and oxidation-reduction processes. KEGG analysis revealed enrichment in glucose and amino acid metabolism pathways, while intron retention and alternative 3′ splice site events accounted for over 80% of alternative splicing events.

Conclusion

The development of S. rugosoannulata fruiting bodies primarily involves glucose and amino acid metabolism, with intron retention and alternative 3′ splice site being the two main types of alternative splicing events playing important roles in development and maturation. This first systematic transcriptome study of S. rugosoannulata provides clues for understanding the molecular regulation of fruiting body development and supports future functional gene identification.

Published in:Frontiers in Genetics,
Study Type:Transcriptome Analysis Study,
Source: PMID: 35903349, DOI: 10.3389/fgene.2022.924050