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Transcriptome Analysis and Its Application in Identifying Genes Associated with Fruiting Body Development in Basidiomycete Hypsizygus marmoreus

Summary

This research analyzed the genetic mechanisms controlling how mushrooms develop from simple fungal threads into mature mushrooms. By studying gene activity at different growth stages, researchers identified key genes and cellular processes that control mushroom formation. This knowledge has several practical implications: • Could help improve commercial mushroom cultivation techniques • May lead to better yields and quality in mushroom farming • Provides insights that could help cultivate other edible mushroom species • Could contribute to developing new strains with enhanced properties • Helps understand fundamental biological processes in fungi

Background

Hypsizygus marmoreus is an important edible mushroom species in Asia, but the molecular and genetic basis of its mushroom morphogenesis remains largely uncharacterized. Understanding the mechanisms of fruit body development is crucial for commercial cultivation and scientific research.

Objective

To elucidate the mechanisms of fruit body development in H. marmoreus by analyzing transcriptomes from four developmental stages and identifying genes associated with fruiting body formation.

Results

The study identified key pathways and genes involved in different developmental stages. The mycelium pigmentation stage was associated with MAPK, cAMP, and blue light signal transduction pathways. The transition from H-M to H-V showed changes in two-component system members. During H-V to H-P transition, stress signals associated with MAPK, cAMP and ROS signals were important inducers. Nitrogen starvation and mTOR signaling pathway were found to be crucial for fruit body maturation.

Conclusion

The study provides the first comprehensive transcriptome analysis of H. marmoreus development, revealing complex molecular mechanisms involving light response, stress response, and nitrogen metabolism during different developmental stages. The identified genes and pathways provide valuable insights into mushroom morphogenesis and may help improve commercial cultivation.
  • Published in:PLoS One,
  • Study Type:Transcriptome Analysis,
  • Source: 10.1371/journal.pone.0123025
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