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Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis

Summary

This study explores how mushrooms break down cellulose to grow. Researchers found that a protein called FfMYB15 acts as a master switch that turns on the gene for an enzyme (cellulase) needed to digest cellulose in the Flammulina filiformis mushroom. By controlling this enzyme, FfMYB15 helps the mushroom grow faster and more efficiently on cellulose-rich materials used in cultivation.

Background

Cellulose degradation is essential for mushroom mycelial growth and affects yield in Flammulina filiformis cultivation. While cellulase genes have been detected in mushrooms, the transcriptional regulatory mechanisms controlling cellulose degradation remain poorly understood. This study investigates the role of MYB transcription factors in regulating cellulase gene expression.

Objective

To identify and characterize FfMYB15 transcription factor and its role in regulating the cellulase gene FfCEL6B during mycelial growth of F. filiformis. The study aims to clarify the transcriptional regulation mechanism of cellulose degradation in industrially cultivated mushrooms.

Results

FfCEL6B positively regulated mycelial growth, with expression patterns showing 0.953 correlation with FfMYB15 on 0.9% cellulose medium. FfMYB15 was localized to the nucleus and functioned as a transcriptional activator. EMSA and dual-luciferase assays demonstrated that FfMYB15 could bind and activate the FfCEL6B promoter by recognizing MYB cis-acting elements.

Conclusion

FfCEL6B plays an active role in mycelial growth of F. filiformis and is regulated by transcription factor FfMYB15. This study provides new insights into the transcriptional regulation mechanism of cellulose degradation during mushroom growth and development.
  • Published in:Microbial Cell Factories,
  • Study Type:Experimental Research,
  • Source: PMID: 36253826, DOI: 10.1186/s12934-022-01932-z
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