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The Promoting Mechanism of the Sterile Fermentation Filtrate of Serratia odorifera on Hypsizygus marmoreus by Means of Metabolomics Analysis

Summary

Researchers discovered that a type of bacteria called Serratia odorifera promotes the growth of a popular edible mushroom (Hypsizygus marmoreus) through chemical signal molecules. By analyzing the mushroom’s metabolism when exposed to these bacterial signals, scientists found that the bacteria enhance the mushroom’s ability to process carbohydrates and generate energy, leading to faster mycelial growth and better fruiting. These findings could help improve mushroom farming practices by reducing growing time and increasing yields.

Background

Hypsizygus marmoreus is a commercially important edible mushroom with high nutritional value. Previous research identified Serratia odorifera as a symbiotic bacterium that promotes H. marmoreus growth, but the specific mechanism remained unclear.

Objective

This study aimed to elucidate the growth-promoting mechanism of S. odorifera on H. marmoreus by examining the effects of sterile fermentation filtrate and quorum sensing signaling molecules using metabolomics analysis.

Results

Exogenous addition of HZSO-1 filtrate and QS signaling molecules significantly promoted H. marmoreus mycelial growth and increased clamp junction numbers. Metabolomic analysis identified 706 metabolites with 140 differentially accumulated metabolites (DAMs) in the HZSO-1 group. The pentose phosphate pathway (PPP) was significantly upregulated, promoting energy production through enhanced carbohydrate and amino acid metabolism.

Conclusion

The sterile fermentation filtrate of S. odorifera promotes H. marmoreus growth by regulating carbohydrate and amino acid metabolism through the pentose phosphate pathway, providing increased energy for mycelial growth and development. These findings reveal the bacterial-fungal interaction mechanism and have practical implications for improving mushroom cultivation.
  • Published in:Biomolecules,
  • Study Type:Experimental Study,
  • Source: 10.3390/biom13121804, PMID: 38136674
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