The Irreversible Loss of a Decomposition Pathway Marks the Single Origin of an Ectomycorrhizal Symbiosis

Summary

This research reveals how certain mushroom species permanently lost their ability to decompose dead plant matter as they evolved to form beneficial partnerships with living plants. This evolutionary change represents a point of no return – once these fungi became dependent on their plant partners, they could no longer go back to living independently. Impacts on everyday life: – Helps explain why many forest mushrooms only grow in association with specific trees – Demonstrates how organisms can become permanently dependent on their partners in nature – Provides insights into the evolution of cooperative relationships between species – Improves our understanding of forest ecosystem functioning – Could inform cultivation practices for economically important mushroom species

Background

Microbial symbioses have evolved repeatedly across the tree of life, but the genetic changes underlying transitions to symbiosis are largely unknown, especially for eukaryotic microbial symbionts. The genus Amanita encompasses over 500 species of mushroom-forming fungi found worldwide, with most species being symbiotic (ectomycorrhizal) but some remaining free-living saprobes.

Objective

To identify both the origins and potential genetic changes maintaining the stability of the ectomycorrhizal mutualism within the genus Amanita by examining the evolution of genes involved in cellulose decomposition and experimentally testing saprotrophic capabilities.

Results

The phylogenetic analysis revealed a single origin of the ectomycorrhizal symbiosis within Amanita. Symbiotic species were found to have lost two critical cellulase genes (endoglucanase and cellobiohydrolase) while retaining beta-glucosidase in many cases. Growth experiments confirmed that symbiotic species had lost the ability to grow on complex organic matter but retained the ability to use proteins. The loss of decomposition genes appears to be irreversible and maintains the evolutionary stability of the symbiosis.

Conclusion

The evolution of the ectomycorrhizal symbiosis in Amanita is marked by the irreversible loss of genes required for autonomous growth through decomposition of complex organic matter. This genetic loss prevents reversions to a free-living lifestyle and helps maintain the evolutionary stability of these widespread fungal-plant mutualisms.
Scroll to Top