The Involvement of Glycerophospholipids in Susceptibility of Maize to Gibberella Root Rot Revealed by Comparative Metabolomics and Mass Spectrometry Imaging Joint Analysis

Summary

Maize plants can suffer from a serious root disease called Gibberella root rot caused by a fungus. Researchers studied two types of maize—one resistant and one susceptible to this disease—and found that certain fatty compounds called lysophospholipids are more abundant in the susceptible plants. When these compounds build up, they damage plant cells and help the fungus spread. The resistant plants can break down these harmful compounds more effectively. This discovery could help plant breeders create maize varieties that resist this damaging disease.

Background

Gibberella root rot (GRR), caused by Fusarium graminearum, is a major threat to maize production. The molecular mechanisms underlying maize’s response to GRR are not fully understood. Multi-omics approaches incorporating metabolomics can reveal insights into maize-pathogen interactions.

Objective

To identify specific metabolites associated with GRR resistance or susceptibility in maize using comparative metabolomics and mass spectrometry imaging. To characterize the role of glycerophospholipid metabolism in maize resistance to Gibberella root rot.

Results

Glycerophospholipid metabolism was highly associated with GRR resistance/susceptibility, with 35 of 44 significant lipid changes being lysophospholipids. Susceptible line 335M showed significantly higher accumulation of lysophosphatidylcholine (lysoPC) and lysophosphatidylethanolamine (lysoPE) with elevated phospholipase gene expression. Resistant line W438 showed higher lysophospholipase expression and accumulation of non-toxic sn-glycero-3-phosphocholine.

Conclusion

High levels of lysophospholipids (lysoPC and lysoPE) contribute to GRR susceptibility through toxic effects and ROS accumulation. Maintaining appropriate lysophospholipid concentrations is crucial for signaling pathways that trigger GRR resistance without root damage. These findings provide targets for genome editing to improve maize GRR resistance.
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