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The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus)

Summary

This research studied how temperature and nutrients affect the growth of two types of oyster mushrooms to optimize their cultivation. The findings help improve commercial mushroom production methods. Impact on everyday life: • Provides guidance for more efficient and cost-effective mushroom farming • Enables better use of agricultural waste materials as growth substrates • Helps increase sustainable food production • Makes mushroom cultivation more accessible to small-scale farmers • Contributes to more affordable mushroom availability for consumers

Background

Oyster mushroom (Pleurotus species) belongs to the family Tricholomataceae and is the second most widely cultivated mushroom worldwide after Agaricus bisporus. Pleurotus species are popular due to their simple, low-cost production technology and high biological efficiency. They can efficiently degrade lignin and grow on various agricultural wastes across a wide temperature range. These mushrooms have high economic, ecological and medicinal value, containing important nutrients like proteins, minerals and vitamins comparable to eggs, milk and meat.

Objective

To evaluate and determine optimal temperature and nutritional conditions for mycelium growth of two oyster mushroom species – Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC) – for mushroom spawn production.

Results

PDA and YDA media were most suitable for P. ostreatus growth while all tested media supported P. cystidiosus. Optimal temperature for both species was 28°C. Glucose, sucrose and molasses supported best growth for P. ostreatus while glucose, sucrose and dextrose were optimal for P. cystidiosus. Ammonium chloride was the best nitrogen source. Brown rice was the most favorable grain substrate, while sugarcane residue, acacia sawdust and corn cob were the best lignocellulosic substrates for both species. P. ostreatus showed significantly better mycelium growth than P. cystidiosus across all conditions tested.

Conclusion

The mycelium growth of both oyster mushroom species was significantly affected by temperature and nutritional conditions. Optimal conditions were identified for media type, temperature (28°C), carbon sources (1-5% sucrose for PO, 1-3% for PC), nitrogen sources (0.03-0.09% ammonium chloride for PO, 0.03-0.05% for PC), and substrate materials (brown rice, sugarcane residue, acacia sawdust, corn cob). P. ostreatus consistently demonstrated superior mycelium growth compared to P. cystidiosus under all studied conditions.
  • Published in:Mycobiology,
  • Study Type:Laboratory Research,
  • Source: 10.5941/MYCO.2015.43.1.14
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