The Effect of Natural-Based Formulation (NBF) on the Response of RAW264.7 Macrophages to LPS as an In Vitro Model of Inflammation
- Author: mycolabadmin
- 3/21/2022
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Summary
Researchers tested a natural mixture of five medicinal mushrooms combined with cannabidiol (CBD) on immune cells exposed to bacterial endotoxins. The formulation effectively reduced inflammatory response markers without harming the cells. These findings suggest the mixture could potentially be used to treat inflammatory conditions like asthma and arthritis, though more research is needed.
Background
Macrophages play a critical role in immune responses but their excessive activation can cause chronic inflammatory diseases such as asthma, tuberculosis, hepatitis, and inflammatory bowel disease. Medicinal mushrooms and cannabidiol possess proven immunomodulatory properties through various bioactive compounds including polysaccharides, triterpenoids, and other metabolites.
Objective
To evaluate the in vitro anti-inflammatory effects of a natural-based formulation (NBF) containing five medicinal mushrooms and cannabidiol (CBD) on RAW264.7 macrophages exposed to lipopolysaccharide (LPS), a known inducer of inflammatory responses.
Results
NBF completely inhibited LPS-induced elevation of IL-6, CCL5, COX-2, and nitrite levels. IL-1β and TNF-α release were inhibited by 3.9-fold and 1.5-fold respectively. NBF significantly increased mRNA expression of TLR2 and TLR4 without affecting NF-κB expression. No cytotoxic effects were observed as assessed by LDH release.
Conclusion
The NBF demonstrates potent anti-inflammatory and immunomodulatory effects capable of attenuating multiple pro-inflammatory markers in LPS-stimulated macrophages. These findings suggest NBF may be a candidate for treating acute and chronic inflammatory diseases, though further in vivo studies are warranted to establish clinical applicability and safety.
- Published in:Journal of Fungi (Basel),
- Study Type:In Vitro Cell Culture Study,
- Source: PMID: 35330323