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The Complete Mitochondrial Genome of the Edible Mushroom Grifola frondosa

Summary

This research decoded the complete mitochondrial DNA sequence of the maitake mushroom (Grifola frondosa), providing important genetic information about this valuable edible and medicinal fungus. The study revealed one of the largest mitochondrial genomes among similar mushroom species and helped clarify its evolutionary relationships with other fungi. Impacts on everyday life: • Helps improve cultivation methods for maitake mushrooms, making them more available as food • Aids in quality control and authentication of maitake products in the market • Contributes to better understanding of mushroom evolution and diversity • Supports development of new medicinal applications from maitake • Assists in protecting and preserving authentic maitake mushroom strains

Background

Grifola frondosa, commonly known as maitake, is a delicious culinary-medicinal mushroom rich in bioactive compounds like polysaccharides, proteins, and nonvolatile taste components. Mitochondria and mitochondrial DNA play crucial roles in energy generation, aging, and other physiological processes. While mitochondrial DNA is widely used for phylogeny, evolution, and strain discrimination in fungi, there was previously no information available about the mitochondrial genome of G. frondosa.

Objective

To determine and analyze the complete mitochondrial genome sequence of Grifola frondosa using Illumina sequencing technology.

Results

The circular mitochondrial genome was 197,486 bp in length with a GC content of 25.01%, making it one of the largest mitochondrial genomes in the order Polyporales. The genome contained 108 protein-coding genes, including 13 conserved protein genes, 69 hypothetical protein genes, 24 tRNA genes, 1 ribosomal protein s3 gene, and 1 DNA polymerase gene. The researchers identified 35 Group I introns, 1 Group II and 5 unclassified introns across 8 genes.

Conclusion

The complete mitochondrial genome of G. frondosa provides new insights into genetic information and evolution for further studies. Phylogenetic analysis showed G. frondosa clustered together with Sparassis crispa, Laetiporus sulphureus, Wolfiporia cocos, and Taiwanofungus camphoratus, consistent with previous taxonomic studies.
  • Published in:Mitochondrial DNA Part B, Resources,
  • Study Type:Genomic Analysis,
  • Source: 10.1080/23802359.2021.1917312
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