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The complete mitochondrial genome of medicinally important wood-decaying fungus Tyromyces fissilis within the family Incrustoporiaceae, Polyporales

Summary

Scientists have sequenced the complete genetic blueprint of a wood-rotting mushroom called Tyromyces fissilis for the first time. This fungus is medicinally important and has the ability to break down wood and produce therapeutic compounds. By analyzing its mitochondrial genome and comparing it with related fungi, researchers found that it is most closely related to another fungus called Phlebia radiata and belongs to a previously understudied fungal family. This genetic information will help scientists better understand how these fungi are related and may lead to new medical applications.

Background

Tyromyces fissilis is a globally renowned white-rot basidiomycete with significant potential for lignin degradation and medicinal applications. The fungus has been found to contain lanostane and rearranged lanostane carboxylic acids with potential therapeutic value. However, its mitochondrial genome has received comparatively little attention in the scientific literature.

Objective

This study aimed to sequence and characterize the complete mitochondrial genome of T. fissilis using next-generation sequencing technology. The researchers also conducted phylogenetic analysis to delineate the evolutionary relationships of T. fissilis with other Basidiomycete species, particularly within the Polyporales order.

Results

The complete mitochondrial genome of T. fissilis NEFU_01 spans 163,380 base pairs and comprises 15 protein-coding genes, 24 transfer RNAs, and 2 ribosomal RNAs. The genome encodes 28 LAGLIDADG and 10 GIY-YIG homing endonucleases, with a GC content of 25.95%. Phylogenetic analysis revealed T. fissilis as a basal taxonomic unit within Incrustoporiaceae, with Phlebia radiata as its closest sister species.

Conclusion

This study presents the first complete mitochondrial genome sequence for the family Incrustoporiaceae. The findings establish phylogenetic relationships between T. fissilis and related Polyporales fungi, contributing to understanding of fungal evolution and taxonomy. This mitochondrial genome data enriches the diversity of basidiomycete mitochondrial genomes and provides a foundation for further evolutionary and taxonomic studies.
  • Published in:Mitochondrial DNA B Resources,
  • Study Type:Genomic Analysis,
  • Source: 10.1080/23802359.2025.2478128, PMID: 40099265
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