Sustainable Recycling of Mushroom Residue as an Effective Substitute for Cotton Hull Waste in Volvariella volvacea Cultivation: Evidence from Physicochemical and Microbiome Analyses

Summary

This research shows that mushroom waste left over from growing mushrooms can be successfully recycled as a growing medium for straw mushrooms, similar to the traditional cotton hull waste currently used. The recycled mushroom residue produces mushrooms of equivalent quality and quantity but costs much less to purchase. By analyzing the microscopic bacterial communities in the compost, scientists found that different bacteria help break down the organic matter in both types of substrates, supporting efficient mushroom growth.

Background

Mushroom residue (MR) is extensively produced during industrialized mushroom cultivation, with approximately 3-5 kg of MR generated per kilogram of fresh mushrooms. Cotton hull waste (CW) is the traditional substrate for Volvariella volvacea cultivation in China. This study investigates the potential of recycling mushroom residue as a sustainable alternative substrate.

Objective

To compare MR-based and CW-based cultivation formulas for V. volvacea with respect to physicochemical characteristics, bacterial community composition, and functional dynamics during substrate fermentation and composting.

Results

Xylanase production was higher in MR formula while CMCase was higher in CW formula. Biological efficiency was comparable between formulas, but MR cost was significantly lower (75 CNY/t vs 1200 CNY/t). Bacterial community analysis revealed distinct patterns with Bacillota dominating MR samples and Pseudomonadota predominating in CW samples, with carbohydrate and amino acid metabolism remaining active throughout fermentation.

Conclusion

Mushroom residue represents an economically viable and environmentally sustainable alternative to cotton hull waste for V. volvacea cultivation, with comparable biological efficiency but substantially reduced material costs. The study demonstrates the feasibility of sustainable recycling of spent mushroom substrates through microbiome-driven composting optimization.
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