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Structure and Bioactivity of Intracellular and Extracellular Polysaccharides of Trametes lactinea Mycelium

Summary

Researchers extracted and studied polysaccharides from Trametes lactinea mushroom mycelium grown in liquid culture. They found that these polysaccharides have strong antioxidant properties and can boost immune cell function, with the intracellular polysaccharide IP-3 showing the most promising results. These findings suggest that polysaccharides from mushroom fermentation could have potential health benefits and could be developed into medicinal supplements.

Background

Trametes lactinea is a macro-fungus with medicinal potential. Previous studies have shown that T. lactinea polysaccharides possess antioxidant, anti-inflammatory, and immunomodulatory properties. However, detailed structural characterization and bioactivity comparison of intracellular versus extracellular polysaccharides from liquid fermentation remain limited.

Objective

This study aimed to analyze the structures of intracellular and extracellular polysaccharides isolated from T. lactinea liquid fermentation and investigate their antioxidant, antibacterial, and immunomodulatory properties to support development and utilization of these polysaccharides.

Results

IP-3 was the only component with a triple-helix structure and the highest molecular weight (120,686 Da), flavonoid, and phenolic content. All components showed strong ABTS and DPPH radical scavenging abilities, with weak hydroxyl radical and iron reduction activity. Limited antibacterial effects were observed against tested bacteria, with IP-3 showing the strongest activity. All components promoted RAW264.7 cell proliferation and enhanced phagocytic activity, with dose-dependent increases in NO, TNF-α, and IL-6 secretion.

Conclusion

The intracellular and extracellular polysaccharides from T. lactinea mycelial liquid fermentation demonstrate strong antioxidant and immunomodulatory activities, with IP-3 displaying the most potent bioactivity. These findings support the potential development and utilization of T. lactinea polysaccharides from liquid fermentation for medicinal and nutraceutical applications.
  • Published in:Microorganisms,
  • Study Type:Experimental Research Study,
  • Source: PMID: 39065199, DOI: 10.3390/microorganisms12071431
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