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Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation

Summary

This research focused on optimizing the production of an important industrial enzyme called laccase using agricultural waste (sugarcane bagasse) and fungi. The process also helped break down tough plant materials into more useful forms. This has practical implications for several industries and everyday life: • More efficient and sustainable production of enzymes used in food, beverages, paper and textile industries • Converting agricultural waste into valuable products instead of burning or disposal • Development of greener industrial processes that use less harsh chemicals • Potential applications in biofuel production by helping break down plant materials • Environmental benefits through reduced agricultural waste and more sustainable manufacturing processes

Background

Laccases are blue multicopper oxidases able to oxidize phenolic compounds and, with mediators, also non-phenolic compounds. They are produced by fungi, especially white-rot basidiomycetes, and their production is affected by carbon/nitrogen sources, copper, and organic inducers. Laccases have applications in food, beverages, pulp/paper, textile industries, nanotechnology, bioremediation and synthetic chemistry.

Objective

To optimize fermentation conditions for laccase production and evaluate delignification of sugarcane bagasse through solid-state fermentation using a selected strain of Pleurotus ostreatus (Pl 22 Em).

Results

Maximum laccase activity of 151.6 U/g was achieved after 5 days under optimized conditions (yeast extract 6.4 g/L, Cu2+ 172.6 μM, ferulic acid 1.86 mM). Lignin content in sugarcane bagasse was reduced from 31.89% to 26.36% after 5 days and to 20.79% after 15 days of biological treatment.

Conclusion

The strain produced high levels of laccase activity compared to previous reports. Yeast extract as nitrogen source increased production 5.7-fold versus ammonium sulfate. The process achieved moderate delignification efficiency, with highest rate between days 5-10. The optimized conditions provide a promising system for laccase production from agricultural waste.
  • Published in:BioMed Research International,
  • Study Type:Laboratory Research,
  • Source: 10.1155/2015/181204
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