Stable Reference Gene Selection for Ophiocordyceps sinensis Gene Expression Studies Under Different Developmental Stages and Light-Induced Conditions
- Author: mycolabadmin
- 2023-04-20
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Summary
Background
Chinese cordyceps (Ophiocordyceps sinensis) is a traditional Chinese medicine formed by an entomopathogenic fungus that parasitizes insect larvae. The formation process includes asexual proliferation in host hemolymph and sexual development through fruiting body formation. Understanding the molecular mechanisms requires reliable reference genes for RT-qPCR analysis, but no stable reference genes have been reported for O. sinensis fruiting body development stages.
Objective
To identify and validate stable reference genes for RT-qPCR analysis of O. sinensis under different developmental stages and light conditions. The study aimed to evaluate 10 candidate reference genes during asexual reproduction, fruiting body development, and light-induced conditions.
Results
Conclusion
- Published in:PLOS One,
- Study Type:Laboratory Research,
- Source: 10.1371/journal.pone.0284486