Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites

Summary

Researchers tested whether fermenting hemp plant waste with different mushroom types could make it a better food source for black soldier fly larvae, which produce valuable protein for animal feed. They found that different mushroom species had different effects: Ganoderma lucidum significantly increased the amount of larvae produced, while Trichoderma reesei increased beneficial cannabinoid content. Pleurotus ostreatus and Hypsizygus ulmarius removed unwanted cannabinoids from the waste. This approach offers a promising way to turn hemp waste into high-quality insect feed while controlling the levels of bioactive compounds.

Background

Hemp cultivation generates significant plant waste containing bioactive secondary metabolites like cannabinoids, flavonoids, and terpenes. Solid-state fermentation (SSF) can improve the nutritional quality of low-value substrates. Black soldier fly larvae (BSFL) have shown potential for converting organic waste into valuable biomass while maintaining feed safety.

Objective

To evaluate whether SSF pre-treatment of hemp waste with different fungal species enhances nutritional value for black soldier fly larvae and determine how fermentation alters the composition of hemp secondary metabolites including cannabinoids, flavonoids, and terpenes.

Results

G. lucidum fermentation significantly increased BSFL dry yield (4.54g vs 2.86g in untreated). T. reesei increased CBD and Δ9-THC while decreasing acidic cannabinoids. P. ostreatus and H. ulmarius effectively removed cannabinoids. Flavonoids and terpenes were substantially removed through autoclaving and fermentation processes.

Conclusion

SSF of hemp waste with different fungal species offers complementary benefits: G. lucidum enhances BSFL growth through improved carbon accessibility, while T. reesei enriches cannabinoid profiles. PO and HU effectively reduce secondary metabolites. SSF provides a viable approach to enhance nutritional value and/or reduce bioactive compounds in hemp waste for sustainable feed applications.
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