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SNP and SCAR Markers for Specific Discrimination of Antler-Shaped Ganoderma lucidum

Summary

This research developed new genetic tools to identify special antler-shaped Ganoderma lucidum mushrooms, which are valued in traditional Asian medicine. The study found specific DNA markers that can reliably distinguish these rare antler-shaped mushrooms from common kidney-shaped varieties. Impacts on everyday life: • Helps ensure consumers get genuine antler-shaped Ganoderma products • Enables better quality control in medicinal mushroom production • Supports preservation of valuable medicinal mushroom strains • Aids development of improved cultivation methods • Allows faster and more accurate identification of mushroom varieties

Background

Ganoderma lucidum has been used in traditional medicine for thousands of years in East Asian countries. Antler-shaped G. lucidum is a rare variant known for containing larger amounts of β-D-glucans and triterpenoids than kidney-shaped G. lucidum, with potentially stronger pharmacological activity. However, there have been no specific markers for identifying antler-shaped strains.

Objective

To identify single nucleotide polymorphism (SNP) and sequence characteristic amplification region (SCAR) markers for specific identification of antler-shaped Ganoderma lucidum strains.

Results

An SNP was found only in antler-shaped G. lucidum strains at position 456 bp, which could be digested by HinfI restriction enzyme. Two specific DNA bands (273-bp and 994-bp) were identified in RAPD analysis unique to antler-shaped strains. Based on these, two sets of specific primer pairs were designed that produced specific bands only in antler-shaped strains.

Conclusion

The developed SNP and SCAR markers successfully enable specific identification of antler-shaped G. lucidum strains. These molecular markers will be helpful for genetic identification of morphological characteristics of G. lucidum and protection of antler-shaped strains.
  • Published in:Microorganisms,
  • Study Type:Laboratory Research,
  • Source: 10.3390/microorganisms7010012
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