MYCOLab Ai Logo - gold and army green

Selenium Polysaccharide SPMP-2a from Pleurotus geesteranus Alleviates H2O2-Induced Oxidative Damage in HaCaT Cells

Summary

This research examined how a special compound called SPMP-2a, extracted from mushrooms enriched with selenium, can protect human skin cells from damage caused by harmful oxidative stress. The compound works by boosting the cells’ natural antioxidant defenses and preventing cell death. This has important implications for everyday life: • Could lead to new skincare products that better protect against environmental damage • May help develop treatments for conditions related to oxidative stress • Shows potential for natural antioxidant supplements from mushroom sources • Demonstrates how combining selenium with natural compounds can enhance health benefits • Could inspire new approaches to preventing aging-related cellular damage

Background

Reactive oxygen species (ROS) like superoxide anion free radical, hydroxyl free radical, and hydrogen peroxide can cause oxidative stress when production exceeds scavenging ability. This leads to inflammation, aging, cardiovascular diseases and cell apoptosis. Fungal polysaccharides have shown ability to scavenge free radicals and inhibit lipid peroxidation. Selenium is an essential trace element that constitutes active centers of oxidases and protects cell membranes.

Objective

To isolate and characterize selenium-enriched polysaccharide SPMP-2a from Pleurotus geesteranus and evaluate its protective effects against hydrogen peroxide-induced oxidative damage in human keratinocytes (HaCaT) cells.

Results

SPMP-2a was identified as an acid selenium polysaccharide with α-D-glucopyranoside bond and molecular weight of 3.32 × 104Da. SPMP-2a treatment significantly improved cell viability, reduced nuclear condensation and cell apoptotic rates in H2O2-damaged HaCaT cells. It increased SOD and CAT enzyme activities while reducing ROS content. Western blot analysis showed SPMP-2a increased Bcl-2 protein expression levels.

Conclusion

SPMP-2a demonstrated protective effects against H2O2-induced oxidative damage in HaCaT cells by improving cellular antioxidant enzyme activities, reducing ROS levels, and increasing Bcl-2 gene expression levels, thereby reducing cell apoptosis. This suggests potential applications for SPMP-2a in preventing oxidative stress-related cellular damage.
  • Published in:BioMed Research International,
  • Study Type:Laboratory Research,
  • Source: 10.1155/2017/4940384
Scroll to Top