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Selective Homologous Expression of Recombinant Manganese Peroxidase Isozyme of Salt-Tolerant White-Rot Fungus Phlebia sp. MG-60, and Its Salt-Tolerance and Thermostability

Summary

Scientists studied a special fungus from mangrove forests that can break down tough plant materials in salty environments. They created engineered versions that produce three different types of a cleanup enzyme called manganese peroxidase. One version of this enzyme was found to actually work better in salty conditions and remain stable at high temperatures, making it potentially useful for industrial applications in harsh environments.

Background

Phlebia sp. MG-60 is a salt-tolerant white-rot fungus isolated from mangrove forests that expresses three manganese peroxidase (MnP) isozymes. Previous studies showed differential regulation of these isozymes under hypersaline conditions, but their biochemical properties remained unknown due to purification difficulties.

Objective

To establish forced expression transformants of three MnP isozymes (MGMnP1, MGMnP2, MGMnP3), enable isozyme-selective expression and simple purification in high-nitrogen medium, and investigate the salt-tolerance and thermostability of these recombinant enzymes.

Results

All three MnP isozymes were successfully expressed and purified to apparent homogeneity. MGMnP1 showed highest activity accumulation and displayed remarkable salt tolerance, with activity enhanced by high NaCl concentrations (200-1200 mM). MGMnP1 also demonstrated superior thermostability, retaining 50% activity at 60°C compared to rapid inactivation of other isozymes.

Conclusion

The selective expression system in high-nitrogen medium enables simple purification of individual MnP isozymes and reveals distinct biochemical properties. MGMnPs exhibit significantly higher salt tolerance than P. chrysosporium MnP, suggesting adaptation to chloride-rich mangrove environments, with MGMnP1 being particularly suited for applications in saline conditions.
  • Published in:Journal of Microbiology and Biotechnology,
  • Study Type:Original Research,
  • Source: 34949746
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