Selection and Evaluation of Reference Genes for qRT-PCR in Inonotus obliquus
- Author: mycolabadmin
- 2025-01-29
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Summary
This research focuses on improving the accuracy of genetic studies in the medicinal mushroom Inonotus obliquus (Chaga). The scientists identified the most reliable genes to use as reference points when studying how other genes work in this fungus. This is important because it helps ensure that future research on this medicinal mushroom’s beneficial properties is more accurate and reliable.
Impacts on everyday life:
• Enables more accurate research into Chaga mushroom’s health benefits
• Helps improve understanding of how medicinal mushrooms produce beneficial compounds
• Contributes to developing better medicinal mushroom products
• Supports more efficient production of natural health supplements
• Advances our ability to study and utilize natural medicines
Background
Inonotus obliquus is a rare and edible medicinal fungus containing various bioactive compounds including polysaccharides, obliquin, oxidized triterpenoids, steroids, polyphenols, and low molecular weight components. Real-time quantitative reverse transcription PCR (RT-qPCR) is a highly sensitive tool for studying gene expression, but requires appropriate reference genes for accurate results.
Objective
To systematically evaluate and identify suitable reference genes for RT-qPCR analysis in Inonotus obliquus under different experimental conditions including varying carbon sources, nitrogen sources, growth factors, pH levels, temperatures, strains, and growth stages.
Results
Under varying carbon sources, VPS showed good stability. RPB2 was most stable for different nitrogen sources. PP2A showed highest stability with different growth factors. UBQ was most stable across pH values. RPL4 demonstrated highest stability at different temperatures. RPL2 was most stable among different strains. VAS showed greatest stability across growth stages. Overall, VPS emerged as the most recommended reference gene for the entire sample set.
Conclusion
The study successfully identified optimal reference genes for RT-qPCR analysis in I. obliquus under various experimental conditions. Different conditions require specific reference genes, with VPS being the most broadly applicable. This work provides a foundation for accurate gene expression analysis in I. obliquus research.
- Published in:Frontiers in Microbiology,
- Study Type:Laboratory Research,
- Source: 10.3389/fmicb.2025.1500043