Saprotrophic Arachnopeziza Species as New Resources to Study the Obligate Biotrophic Lifestyle of Powdery Mildew Fungi
- Author: mycolabadmin
- 10/3/2025
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Summary
Scientists have created a new way to study powdery mildew fungus, which causes widespread plant disease but cannot normally be studied in the laboratory. They identified a related fungus species (Arachnopeziza) that can grow in culture and can be genetically modified. By sequencing the DNA of these two Arachnopeziza species and developing methods to alter their genes, researchers have created a practical tool to understand how powdery mildew becomes dependent on its plant host, potentially leading to better disease control strategies.
Background
Powdery mildew fungi are obligate biotrophic plant pathogens that cannot be cultivated in vitro or genetically modified due to their dependence on living host plant tissue. The family Arachnopezizaceae, comprising saprotrophic fungi, are the closest known extant relatives of powdery mildew fungi and may provide insights into the genetic basis of obligate biotrophy.
Objective
To establish Arachnopeziza species as a model system for studying powdery mildew genetics by generating chromosome-level genome assemblies, developing cultivation protocols, and establishing genetic transformation methods for two Arachnopeziza species.
Results
Both A. aurata and A. aurelia genomes contain 16 chromosomes with compact sizes (43.1 and 46.3 Mbp respectively) and low repeat content (<5%), showing evidence of repeat-induced point mutation. Both species grew readily on standard fungal media and in liquid culture, and were successfully transformed with fluorescent protein and antibiotic resistance markers.
Conclusion
Arachnopeziza species represent a tractable experimental system that can overcome limitations in studying powdery mildew genetics by serving as a proxy to investigate molecular functions of powdery mildew proteins while avoiding the challenges of cultivating obligate biotrophic pathogens.
- Published in:Molecular Ecology Resources,
- Study Type:Genomic and Molecular Characterization Study,
- Source: PMID: 41044902, DOI: 10.1111/1755-0998.70045