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RNA Polymerase II Second Largest Subunit Molecular Identification of Boletus griseipurpureus Corner from Thailand and Antibacterial Activity of Basidiocarp Extracts

Summary

This research examined an edible mushroom found in Thailand called Boletus griseipurpureus, studying both its genetic makeup and potential medical benefits. The study found that extracts from this mushroom can effectively fight against harmful bacteria, including antibiotic-resistant strains. This discovery is particularly significant for public health and natural medicine development. Impacts on everyday life: • Identifies a natural food source with potential antibacterial properties • Suggests new possibilities for developing natural antibiotics from mushrooms • Contributes to our understanding of safe, edible wild mushrooms • Opens new avenues for natural health supplements • Provides scientific validation for traditional uses of mushrooms in medicine

Background

Boletus griseipurpureus Corner is an edible ectomycorrhizal mushroom with unclear taxonomic boundaries and a distinctive bitter taste. The mushroom is protein-rich and low in fat. Previous internal transcribed spacer (ITS) analysis revealed a monophylogenetic clade, but this alone cannot indicate population differences at the subspecies level. The RNA polymerase II second largest subunit (RPB2) was chosen for this study as it is more variable and informative than ITS regions.

Objective

The study aimed to identify genetic variation in B. griseipurpureus collected from different regions of Thailand and evaluate potential antibacterial activities of its extracts.

Results

All collected samples formed a monophyletic clade closely related to the Boletus group of polypore fungi. The crude methanol extract of basidiomes showed stronger inhibition of bacterial growth in vitro compared to crude ethyl acetate extract. The minimal inhibition concentration of the crude methanolic extract was 31 g/L.

Conclusion

Basidiomes collected from the four locations in Thailand demonstrated low genetic variation, and their extracts successfully inhibited the growth of all tested bacteria. The findings suggest potential health benefits of this edible species that warrant further investigation.
  • Published in:Jundishapur Journal of Microbiology,
  • Study Type:Laboratory Research,
  • Source: 10.5812/jjm.15552
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