Recovery of Filter Paperase from Mouldy Rice Husk in Solid State Fermentation by Aspergillus protuberus
- Author: mycolabadmin
- 2024-06-21
- View Source
Summary
This research focused on optimizing the extraction of an important industrial enzyme called filter paperase from rice husk using a fungal fermentation process. The findings are significant for making enzyme production more sustainable and cost-effective.
Impacts on everyday life:
• Enables more efficient use of agricultural waste like rice husk
• Helps reduce production costs of industrial enzymes used in paper, textile and biofuel industries
• Contributes to more sustainable and environmentally-friendly manufacturing processes
• Could lead to cheaper consumer products that rely on these enzymes
• Demonstrates ways to add value to agricultural waste materials
Background
Recovery of enzymes like FPase (filter paperase) or exoglucanase from fermented substrate is important for sustainable enzyme production, but optimization studies in this area are scarce. Solid state fermentation (SSF) is gaining interest for cost-effective enzyme production due to low capital investment and easy handling, but enzyme leaching from mold substrate remains a key challenge that needs optimization.
Objective
The study aimed to standardize multiple parameters (selection of solvent, solvent volume, soaking time, leaching conditions and number of washes) to extract maximum amount of FPase from fermented rice husk using Aspergillus protuberus.
Results
Citrate phosphate buffer (0.02 M, pH 5.0) proved to be the best solvent for maximum FPase recovery. Two washes with citrate phosphate buffer each by shaking for 60 minutes in a ratio of 1g rice husk to 5ml buffer achieved 88% recovery efficiency of FPase from the fermented rice husk. Maximum FPase activity of 2.67 FPU/g was obtained from 1-day fermented husk.
Conclusion
This is the first report on leaching of FPase from rice husk in SSF by A. protuberus. The optimized parameters enabled recovery of approximately 90% of FPase from fermented rice husk. The findings provide important insights for downstream processing and enzyme recovery in SSF systems.
- Published in:Current Research in Microbial Sciences,
- Study Type:Laboratory Research,
- Source: 10.1016/j.crmicr.2024.100254