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Production of Oxidative and Hydrolytic Enzymes by Coprinus cinereus from Sisal Wastes Supplemented with Cow Dung Manure

Summary

This research demonstrates how mushrooms can be used to convert agricultural waste into valuable products. The study focused on growing edible mushrooms on sisal waste while studying the enzymes they produce during growth. This has important implications for sustainable agriculture and waste management. Impacts on everyday life: – Provides a way to convert agricultural waste into nutritious food (mushrooms) – Offers sustainable solutions for managing agricultural waste – Creates potential new sources for industrial enzymes – Demonstrates environmentally friendly farming practices – Could help reduce waste disposal costs for farmers and industries

Background

Enormous quantities of organic wastes from agricultural and food processing industries which are lignocellulosic in nature are generated annually. In Tanzania, high quantities of solid sisal wastes are produced annually as only 2% of the plants are used for sisal fibre production while the remaining 98% are commonly disposed of raw into the environment. Edible mushroom cultivation represents one of the most economically viable processes for bioconversion of these lignocellulosic wastes.

Objective

To determine the profile of oxidative and hydrolytic enzymes during vegetative growth, fruiting, and postharvest stage in three flushes of Coprinus cinereus on solid sisal waste fractions supplemented with cow manure.

Results

Laccase showed the highest activity among the enzymes tested, with peak activity of 39.45 ± 12.05 Ug−1 during mycelia growth on 10% supplemented substrate. Lignin peroxidase exhibited a wave-like pattern with highest activity of 1.93 ± 0.62 Ug−1. The highest CMCase activity was 2.03 ± 0.70 Ug−1, pectinase peaked at 1.90 ± 0.32 Ug−1, and xylanase showed maximum activity of 1.23 ± 0.12 Ug−1. Enzyme activities varied with substrate formulation and growth stage.

Conclusion

Coprinus cinereus can be successfully cultivated on solid sisal waste formulations amended with cow dung manure due to its ability to produce necessary enzymatic activities for lignocellulose degradation and developmental regulation. The pattern of both hydrolytic and oxidative enzyme activities was affected by the substrate composition, growth stage, and mushroom development. The residual spent substrate may serve as a cheap source of lignocellulolytic enzymes for applications in bioremediation and enzyme production.
  • Published in:Biotechnology Research International,
  • Study Type:Experimental Study,
  • Source: 10.1155/2015/650543
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