MYCOLab Ai Logo - gold and army green

PRMT5 promotes cellulase production by regulating the expression of cellulase gene eg2 through histone methylation in Ganoderma lucidum

Summary

Scientists discovered that a protein called PRMT5 controls how much cellulase enzyme the medicinal mushroom Ganoderma lucidum produces. When PRMT5 activates a specific gene called eg2 through a molecular modification of histone proteins, the mushroom produces more cellulase. This enzyme is valuable for breaking down plant waste into useful sugars for industrial and bioenergy applications. This research could help develop better enzyme-producing strains for industries that need cellulase.

Background

Cellulase enzymes degrade cellulose into reducing sugars and have significant applications in industrial production, environmental protection, and bioenergy development. Ganoderma lucidum, a white rot fungus, secretes substantial amounts of cellulase during growth. Post-translational modifications are known to affect cellulase production, and PRMT5 has been implicated in various biological regulatory processes.

Objective

This study investigated whether and how protein arginine methyltransferase 5 (PRMT5) regulates cellulase production in G. lucidum, specifically examining its role in regulating the endoglucanase 2 (eg2) gene through histone methylation mechanisms.

Results

Silencing prmt5 reduced cellulase activity by 23% and mycelial growth rate by 71%, with agricultural waste degradation declining by 60% for corn straw and 51% for corn cob. ChIP-qPCR demonstrated that PRMT5 positively regulates eg2 expression through H4R3me2s modification. eg2 expression directly correlated with cellulase activity, mycelial growth, and agricultural waste degradation capacity.

Conclusion

PRMT5 in G. lucidum promotes cellulase production by positively regulating eg2 expression through histone methylation, specifically H4R3me2s modification. These findings provide a theoretical basis for developing improved cellulase-producing strains for industrial applications and understanding fungal regulatory networks.
  • Published in:Microbial Cell Factories,
  • Study Type:Experimental Research Study,
  • Source: PMID: 40830861
Scroll to Top