Photoregulation of the biosynthetic activity of fungus Inonotus obliquus using colloidal solutions of biogenic metal nanoparticles and low-intensity laser radiation

Summary

Scientists studied how to enhance the medicinal properties of chaga mushroom using tiny metal particles and laser light. They found that adding silver, iron, or magnesium nanoparticles to growing mushroom cultures increased biomass production. When combined with laser treatment, these nanoparticles dramatically boosted the production of beneficial compounds like polysaccharides, flavonoids, and melanin pigments that have health benefits including antioxidant and immune-boosting properties.

Background

Inonotus obliquus (chaga) is a medicinal mushroom containing over 250 bioactive compounds with pharmacological properties. Nanoparticles and laser radiation independently demonstrate effects on fungal development. The combined use of these two factors has not been previously studied in fungal cultivation.

Objective

To investigate the integrated effects of colloidal solutions of biogenic metal nanoparticles (silver, iron, magnesium) and low-intensity laser radiation (488 nm) on the growth characteristics and biosynthetic activity of Inonotus obliquus in vitro cultivation.

Results

Nanoparticles alone increased mycelial growth by 55-60%. Laser irradiation with nanoparticles reduced growth activity by 12.3-35.4%. AgNPs with laser radiation increased intracellular polysaccharides 9.7-fold. MgNPs with laser increased extracellular polysaccharides 20-fold. All photoactivated nanoparticles enhanced flavonoid synthesis and melanin production by 25-140%.

Conclusion

Colloidal solutions of Fe, Ag, and Mg nanoparticles combined with low-intensity laser radiation represent environmentally friendly factors for regulating biosynthetic activity in I. obliquus biotechnology. Different nanoparticle types show distinct effects on polysaccharide localization and secondary metabolite production, suggesting potential for optimization of medicinal compound production.
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