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Partial Purification and Biochemical Evaluation of Protease Fraction (MA-1) from Mycoleptodonoides aitchisonii and its Fibrinolytic Effect

Summary

Scientists have isolated an enzyme from an edible mushroom that shows promise in preventing and treating blood clots. This enzyme, called MA-1, works by breaking down blood clots and preventing new ones from forming, potentially offering a natural approach to preventing cardiovascular diseases. Impacts on everyday life: • Could provide a new natural treatment option for people at risk of blood clots • Demonstrates the potential health benefits of edible mushrooms in preventing cardiovascular disease • May lead to development of safer blood-thinning medications with fewer side effects • Shows promise for creating new functional foods for heart health • Highlights the importance of traditional medicinal mushrooms in modern medicine

Background

Mycoleptodonoides aitchisonii is an edible mushroom belonging to the Climacodontaceae family, found mainly in India and Japan. It has been used as a natural health product and recently succeeded in mass production through artificial cultivation in South Korea. Previous studies have shown various biological effects including anti-asthma, immune-stimulating, anti-obesity, and anti-inflammatory properties.

Objective

To purify and characterize the protease fraction (MA-1) from M. aitchisonii and evaluate its fibrinolytic and antithrombotic effects.

Results

MA-1 was purified with an 89-fold increase in specific activity and 42.5% recovery. It showed optimal activity at 40°C and pH 5.5, with stability between pH 4-11. MA-1 exhibited stronger fibrinolytic activity than plasmin and could hydrolyze fibrinogen chains within 2 hours. The enzyme showed significant antithrombotic effects in animal models without excessive bleeding complications.

Conclusion

M. aitchisonii produces an acid-tolerant and antioxidative chymotrypsin-like fibrinolytic enzyme (MA-1). The findings suggest that M. aitchisonii containing MA-1 could be a beneficial functional material for preventing cardiovascular diseases and their possible complications.
  • Published in:Antioxidants (Basel),
  • Study Type:Laboratory Research,
  • Source: 10.3390/antiox12081558
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