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Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses

Summary

This research discovered that shiitake mushrooms produce oxalic acid, which can effectively kill harmful bacteria that cause plant diseases. The study found that the waste material left after growing shiitake mushrooms contains high levels of this beneficial compound, suggesting a sustainable way to use mushroom byproducts for protecting crops. Impacts on everyday life: • Provides a natural alternative to chemical pesticides for farmers and gardeners • Offers a way to recycle mushroom growing waste into useful products • Could lead to more sustainable and environmentally friendly farming practices • May reduce food waste by protecting crops from bacterial diseases • Demonstrates the additional benefits of mushroom cultivation beyond food production

Background

Lentinula edodes (shiitake mushroom) is known for its nutritional value and medical properties including antitumor, immunopotentiation, and hypoglycemic effects. The mushroom produces antimicrobial compounds like lenthionine, and its spent mushroom substrate (SMS) may contain bioactive compounds with antimicrobial activity. While organic acids from mushrooms have been studied for antioxidant properties, their antimicrobial potential against plant pathogenic bacteria has been less explored.

Objective

To characterize antibacterial substances, particularly oxalic acid, from the culture products of L. edodes and evaluate their biological activity against phytopathogenic bacteria.

Results

Nine organic acids were detected in the culture filtrate, with oxalic acid being the dominant component (50%). Oxalic acid showed the highest antibacterial activity against R. solanacearum and other phytopathogenic bacteria at minimum inhibitory concentrations of 200-250 mg/L. The concentration of oxalic acid in culture filtrate was 708.711 ppm (0.07%). Higher concentrations were found in water extracts from spent mushroom substrate.

Conclusion

Oxalic acid from L. edodes exhibits significant antibacterial activity against various plant pathogenic bacteria. The water extract of spent L. edodes substrate, containing high levels of oxalic acid, shows potential as an eco-friendly control agent for plant diseases.
  • Published in:Mycobiology,
  • Study Type:Laboratory Research,
  • Source: 10.5941/MYCO.2016.44.4.338
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