Optimization of Growth Conditions and Biological Activities of Nepalese Ganoderma lucidum Strain Philippine

Author: mycolabadmin
2021-10-04
View Source

Summary

This research explored how to best grow and utilize the medicinal mushroom Ganoderma lucidum, commonly known as Reishi. Scientists determined optimal growing conditions and compared the beneficial properties of different parts of the mushroom. The study found that both the mushroom’s fruiting body and its root-like structure (mycelia) contain compounds that can fight bacteria and harmful free radicals in the body. Impacts on everyday life: • Provides a scientific basis for more efficient cultivation of medicinal mushrooms • Demonstrates natural alternatives to synthetic antibiotics • Supports the traditional use of Reishi mushroom in health supplements • Shows potential for developing new natural antioxidant products • Helps make medicinal mushroom production more cost-effective

Background

Ganoderma lucidum is a well-known medicinal polypore fungus widely used in East Asia, America, and other countries for its health benefits. It contains over 400 bioactive compounds with various medicinal effects including antitumor, anti-inflammatory, antimicrobial, antifungal, antiviral, and antioxidative properties. While traditionally cultivated through solid-state fermentation taking about six months to form fruiting bodies, alternative rapid cultivation methods are being explored.

Objective

This study aimed to cultivate G. lucidum in various media and determine the optimum growth parameters (pH, temperature, and carbon sources). Additionally, it sought to analyze and compare the phenolic content, flavonoid content, antioxidant activity, and antibacterial activity of extracts from both mycelia and fruiting body cultures.

Results

Optimal growth conditions were found to be pH 4.5-5.5, temperature 28-32°C, with sorbose and trehalose as preferred carbon sources. Both 200 mg/mL and 100 mg/mL concentrations of extracts showed antibacterial activity against all tested bacteria, performing better than control antibiotics. The fruit extract demonstrated higher antioxidant potential (150.6 ± 56.92 mg ascorbic acid equivalent/g extract) compared to mycelial extract (144.28 ± 81.72 mg ascorbic acid equivalent/g extract).

Conclusion

G. lucidum can be optimally cultivated in sawdust substrate and PDA plates under specific conditions. Both mycelia and fruiting bodies showed comparable yields and biological activities. The results indicate that fruiting bodies cultivated in complex dust medium possess higher antioxidant properties than mycelia culture, warranting further investigation for therapeutic applications.

Published in:Advances in Pharmacological and Pharmaceutical Sciences,
Study Type:Laboratory Research,
Source: 10.1155/2021/4888979