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Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor

Summary

This research developed an improved method for producing valuable medicinal compounds from the mushroom Ganoderma lucidum in laboratory conditions. Instead of traditional wood-based cultivation, researchers optimized liquid culture conditions and designed a new bioreactor system that significantly increased production of beneficial compounds. Impacts on everyday life: • Provides a more efficient way to produce natural medicinal compounds • Could help reduce costs and improve availability of mushroom-based medicines • Demonstrates sustainable alternatives to wild mushroom harvesting • Advances technology for producing natural pharmaceutical ingredients • Could lead to more consistent quality in mushroom-based health products

Background

Ganoderma lucidum is a medicinal mushroom containing bioactive triterpenoids and polysaccharides. With increasing market demand, wild resources are limited and cultivated fruiting bodies face quality issues. Traditional cultivation requires extensive wood resources and faces management challenges. Alternative production methods are needed for efficient triterpenoid production.

Objective

To optimize culture conditions for ganoderic acid (GA) production in G. lucidum liquid static culture by investigating effects of nitrogen limitation, carbon supply, static culture volume and air supply. Additionally, to design and test a suitable bioreactor for industrial-scale production.

Results

Under optimized conditions, total yield of five GAs reached 963 mg/L, with GA-T accounting for 75%. Nitrogen limitation and proper static culture volume promoted GA accumulation. Air supply significantly improved production, reaching 986.53 mg/L. The designed bioreactor achieved 856.8 mg/L total GA yield with 5.99% GA content in mycelia, exceeding pharmacopeia requirements by 12-fold.

Conclusion

The study demonstrated successful optimization of culture conditions for GA production in G. lucidum static culture. Air supply was identified as a crucial factor for triterpenoid accumulation. The designed plastic bag bioreactor proved effective for industrial-scale production, offering advantages in sterilization, space utilization, sampling, and cost-effectiveness.
  • Published in:Molecules,
  • Study Type:Laboratory Research,
  • Source: 10.3390/molecules23102563
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