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One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

Summary

This research focused on extracting and purifying two different types of lipase enzymes from a fungus called Schizophyllum commune. These enzymes can break down and modify fats in environmentally friendly ways, offering alternatives to harsh chemical processes used in industry. Impacts on everyday life: – More sustainable and eco-friendly production of ingredients used in foods, cosmetics, and pharmaceuticals – Improved processing of oils and fats for various consumer products – Development of greener industrial processes that reduce environmental impact – Potential cost reduction in manufacturing processes using these enzymes – New ways to produce biodiesel and other biofuels more efficiently

Background

Lipases with unique characteristics are valuable for industrial applications, especially those targeting cost-effectiveness and fewer downstream processes. Filamentous fungi like Schizophyllum commune are suitable for solid state fermentation due to their substrate colonizing mycelium, limited water tolerance and extracellular enzyme production. Lipases are increasingly used to convert water insoluble substrates as eco-friendly alternatives to chemical processes.

Objective

The aims of this research were to optimize fermentation parameters via solid state fermentation and study the performance of one-step partially purified Schizophyllum commune UTARA1 lipases in hydrolysis and esterification processes.

Results

ScLipA showed affinity for long, medium and short chain triglycerides, degrading all tested oils. ScLipB specifically degraded only long chain triglycerides. During esterification, ScLipA synthesized trilaurin and triacetin, while ScLipB specifically formed 2-mono-olein and triacetin. Both ScLipA and ScLipB were determined to be sn-2 regioselective lipases.

Conclusion

The one-step partial purification strategy proved feasible for purifying S. commune UTARA1 lipases with potential industrial applications. The partially purified lipases showed different substrate specificities and regioselectivity, making them valuable for selective hydrolysis of lipids, triglyceride modifications, and biodiesel production.
  • Published in:Molecules,
  • Study Type:Laboratory Research,
  • Source: 10.3390/molecules22122106
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