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On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test

Summary

Scientists developed a quick test to identify two specific types of oyster mushrooms (SPOPPO and ALLERPO) on farms or spawn production facilities. These sporeless mushroom varieties were created to protect workers from respiratory problems caused by mushroom spores. The new LAMP test can identify which strain is being grown in just 30 minutes using simple sample preparation, helping protect the breeding companies’ intellectual property rights from illegal copying.

Background

Protection of intellectual property rights on mushroom varieties is crucial for breeding companies to recover investments. SPOPPO and ALLERPO are two closely related sporeless strains of Pleurotus ostreatus developed to reduce respiratory problems in workers exposed to mushroom spores. Distinguishing these strains in commercial practice is difficult due to minimal phenotypic differences.

Objective

To develop molecular tools for rapid on-site identification and discrimination of two closely related commercial oyster mushroom strains (SPOPPO and ALLERPO) to combat intellectual property infringement.

Results

The MSH4 LAMP assay successfully identified both sporeless strains within 17-24 minutes with high specificity. The AQU07 assay specifically identified ALLERPO while the AQP58 LAMP-CRISPR assay identified SPOPPO. All three assays worked effectively across different matrices with minimal sample requirements (two spawn kernels or one substrate piece sufficient).

Conclusion

Three LAMP-based assays were successfully developed for on-site discrimination of SPOPPO and ALLERPO strains from sporulating varieties and from each other. The method provides rapid identification within 30 minutes using simple extraction procedures, making it suitable for practical application in detecting intellectual property infringement in commercial mushroom production.
  • Published in:Molecular Biology Reports,
  • Study Type:Method Development Study,
  • Source: PMID: 41148377, DOI: 10.1007/s11033-025-11156-0
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