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Oestrogen Detoxification Ability of White Rot Fungus Trametes hirsuta LE-BIN 072: Exoproteome and Transformation Product Profiling

Summary

A type of white rot fungus called Trametes hirsuta can effectively remove harmful oestrogen chemicals from water and soil. The fungus produces special enzymes that break down these hormones into less toxic compounds within just one day. This discovery suggests the fungus could be used as a natural treatment to clean up environmental contamination caused by oestrogens from human waste and pharmaceutical use.

Background

White rot fungi, particularly Trametes species, are effective at degrading xenobiotics including oestrogens, which are widespread environmental contaminants and endocrine disruptors. Oestrone (E1) and 17β-oestradiol (E2) are major endocrine disrupting chemicals that accumulate in soil and water through pharmaceutical use and human/animal excretion, posing risks to human health and ecosystems.

Objective

To investigate the ability of Trametes hirsuta LE-BIN 072 to biotransform oestrone and 17β-oestradiol, identify transformation products, and characterize the secreted proteins (exoproteome) involved in oestrogen detoxification.

Results

Over 90% of E1 and E2 were removed within 24 hours of cultivation. The primary transformation pathway involved oxidative coupling of oestrogens and metabolites forming dimers with reduced biological activity. Three ligninolytic peroxidases (MnP5, MnP7, VP2) were identified as key enzymes, along with aryl-alcohol oxidases. Multiple minor metabolites including estriol (E3) and various hydroxylated and dimeric products were identified.

Conclusion

Trametes hirsuta LE-BIN 072 demonstrates promising potential as a mycodetoxicant for phenol-like steroids in aquatic environments through primarily detoxification mechanisms involving ligninolytic peroxidases and aromatic alcohol oxidases that generate reactive intermediates for oestrogen transformation.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Research,
  • Source: PMID: 39590714
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