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Novel and advanced MNP molecular markers accurately identify the genetic similarity of Hypsizygus marmoreus strains: a comparative evaluation with ISSR and antagonistic methods

Summary

Researchers developed a new genetic fingerprinting method using 369 molecular markers to accurately identify different strains of H. marmoreus mushrooms. By analyzing the DNA sequences of 32 strains, they found that no two strains were genetically identical, with significant diversity patterns observed between white and gray varieties. This new method proved more reliable and efficient than traditional testing approaches, helping prevent the problem of identical mushroom strains being sold under different names.

Background

Hypsizygus marmoreus is an industrially cultivated wood-rotting fungus with significant medicinal value. The expansion of production has created a problem of identical strains being marketed under different names, necessitating accurate and efficient variety identification methods.

Objective

To develop and validate Multiple Nucleotide Polymorphism (MNP) molecular markers for accurate identification of genetic similarity among H. marmoreus strains and compare their effectiveness with ISSR molecular markers and cross-plating antagonistic methods.

Results

No strains exhibited 100% genetic similarity. White strains showed genetic similarity ranging from 11.92% to 88.62% (average 39.64%), while gray strains ranged from 2.71% to 74.53%, indicating greater genetic diversity in gray strains. MNP markers demonstrated superior stability and accuracy compared to antagonistic and ISSR methods.

Conclusion

The MNP molecular marker method based on whole-genome resequencing provides a reliable, efficient, and objective approach for H. marmoreus strain identification and significantly contributes to variety protection and breeding advancement.
  • Published in:Fungal Biology,
  • Study Type:Experimental Research,
  • Source: PMID: 41209487
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