Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants

Summary

Scientists discovered that Italian white truffles (Tuber magnatum) can live inside the roots of common herbaceous plants like sedges, not just the oak and poplar trees where they are typically found. Using special fluorescent probes and advanced microscopy, researchers confirmed the presence of active truffle threads inside these plants, particularly in spring. This discovery challenges our understanding of how truffles interact with their environment and may explain why truffle cultivation has been difficult, suggesting they employ more diverse survival strategies than previously thought.

Background

Tuber magnatum (Italian white truffle) is known as an ectomycorrhizal fungus associating with broadleaf trees, but its mycorrhizae are rarely observed in the field. Recent studies suggest other Tuber species may develop as endophytes in non-ectomycorrhizal plants, but T. magnatum endophytic abilities remain unexplored.

Objective

To investigate potential endophytic interactions of T. magnatum with wild plants using PCR and newly designed Fluorescence In Situ Hybridization (FISH) probes. The study aimed to design specific FISH probes, validate their specificity, and confirm T. magnatum presence in root systems of herbaceous plants.

Results

Molecular analyses detected T. magnatum in 21 of 100 plant samples examined. FISH analysis confirmed the presence of active T. magnatum hyphae inside root systems of Carex pendula. Detection was restricted to spring samples, suggesting seasonal variation in endophytic colonization.

Conclusion

This study provides the first evidence of T. magnatum acting as an endophyte in herbaceous plants, specifically C. pendula. The newly designed, highly specific T. magnatum FISH probes enable further investigation of T. magnatum endophytic tendencies and their influence on truffle biology and life cycle.
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