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Neuroprotective Effects of a Novel Peptide from Lignosus rhinocerotis Against 6-Hydroxydopamine-Induced Apoptosis in PC12 Cells by Inhibiting NF-κB Activation

Summary

This research discovered a new peptide from the medicinal mushroom Lignosus rhinocerotis that could help protect brain cells from damage related to Parkinson’s disease. The peptide works by reducing harmful oxidative stress and preventing cell death through multiple protective mechanisms. This discovery is significant for everyday life in several ways: • Could lead to new natural treatments for Parkinson’s disease with fewer side effects than current medications • Demonstrates the potential of traditional medicinal mushrooms in modern medicine • Provides hope for aging populations at risk of neurodegenerative diseases • Shows promise for developing preventive treatments for brain health • Highlights the importance of natural product research in drug discovery

Background

Parkinson’s disease (PD) affects 2-3% of individuals over age 65 and is the second most common neurodegenerative disease. Oxidative stress is considered an important cause of PD, as the brain is particularly vulnerable to oxidative stress-related damage due to high oxygen requirements but relatively scarce antioxidant enzymes. Conventional treatments like L-DOPA have significant side effects, creating a need for new neuroprotective agents.

Objective

To isolate and characterize a novel peptide (LRP) from Lignosus rhinocerotis and evaluate its neuroprotective effects against 6-OHDA-induced apoptosis in PC12 cells, specifically investigating its impact on oxidative stress and NF-κB pathway activation.

Results

LRP was identified as a 1532 Da peptide containing 15 amino acids. The peptide significantly protected PC12 cells from 6-OHDA-induced damage by reducing ROS production and MDA content while increasing antioxidant enzyme activities (SOD, CAT, GSH-Px). LRP also reduced cell apoptosis by modulating the Bcl-2/Bax ratio and inhibiting Caspase-3/9 activation. The protective effects were mediated through inhibition of NF-κB pathway activation.

Conclusion

LRP demonstrates strong inhibitory effects against 6-OHDA-induced apoptosis in PC12 cells by restoring antioxidant enzyme activities, decreasing ROS levels, adjusting apoptosis-related protein ratios, and inhibiting NF-κB pathway activation. These findings suggest LRP’s potential as a therapeutic agent for treating neurological diseases.
  • Published in:Food Science & Nutrition,
  • Study Type:In Vitro Study,
  • Source: 10.1002/fsn3.3050
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