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Mycelium Growth and Development of Psilocybe spp. Mother Cultures on Agar-Based Media

Summary

This research examines how different growing media affect four types of psilocybin mushrooms. The study found that malt extract agar worked best for some mushroom types while potato-based media worked better for others. Commercial powdered potato media performed poorly, especially for one sensitive strain. These findings help optimize mushroom cultivation for therapeutic and commercial purposes.

Background

The therapeutic potential of psilocybin-producing mushrooms has increased research and commercialization efforts. Psilocybe species are the primary genus of interest due to ease of cultivation and high potency. Standardization of maintenance and cultivation techniques is needed for efficient and consistent production.

Objective

To examine mycelial growth and morphology of four Psilocybe genotypes cultured on different agar-based media. The study aimed to provide foundational information for developing genotype-specific mycelial growth and development practices.

Results

MEA supported superior growth of APE and JMF mycelium, while PDA from raw ingredients favored PA growth. B+ showed vigorous growth on most media treatments, achieving full colonization by day 20. Commercial PDA powder (PDA R) showed poor performance, particularly failing to support APE growth. Significant differences in growth rates and morphologies were observed across different media types and genotypes.

Conclusion

Media selection significantly impacts Psilocybe mycelial growth, with genotype-specific responses. MEA and PDA prepared from raw ingredients proved superior to commercial formulations. Future optimization studies should consider mineral nutrient, vitamin, and amino acid concentrations to develop GMP- and GACP-compliant media formulations for efficient Psilocybe production.
  • Published in:Journal of Fungi (Basel),
  • Study Type:Experimental Study,
  • Source: 10.3390/jof11060450, PMID: 40558962
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