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Multiomics Provides a New Understanding of the Effect of Temperature Change on the Fermentation Quality of Ophiocordyceps sinensis

Summary

This research studied how temperature affects the growth and quality of Ophiocordyceps sinensis, a valuable medicinal fungus. Scientists found that the fungus grows best between 18-23°C, and that temperatures above 28°C damage the fungus by triggering cell death processes. The study identified which genes and metabolites are affected by temperature changes, helping optimize large-scale production of this medicinal fungus.

Background

Ophiocordyceps sinensis is a medicinal fungus with significant nutritional and medicinal value used in traditional Chinese medicine. Temperature is a crucial factor influencing fungal growth and fermentation quality, but limited reports exist on its specific effects on fungal fermentation quality.

Objective

This study aims to determine the optimal culture temperature range for O. sinensis and elucidate the molecular mechanisms underlying temperature-induced changes in fermentation quality through integrated metabolomic and transcriptomic analysis.

Results

The optimal culture temperature range was identified as 18-23°C with maximal growth, sporulation, and antioxidant enzyme activity. At 28°C, genes and metabolites associated with apoptosis and senescence were highly expressed, leading to cell damage and inhibited mycelial growth. Key metabolic pathways enriched included linoleic acid metabolism, arginine and proline metabolism, and lysine degradation.

Conclusion

This study establishes the optimal temperature range for O. sinensis cultivation and demonstrates that temperature stress activates apoptosis and senescence pathways while suppressing normal growth metabolism, providing theoretical support for industrial-scale production.
  • Published in:Journal of Fungi (Basel),
  • Study Type:Experimental Study,
  • Source: 40558916
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