Multi-omics analysis of Taiwanofungus gaoligongensis: effects of different cultivation methods on secondary metabolites
- Author: mycolabadmin
- 8/1/2025
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Summary
This study examined how different growing methods affect the medicinal compounds found in Taiwanofungus gaoligongensis, a rare Chinese medicinal fungus. Researchers discovered that growing this fungus on wood substrates from specific trees significantly increased production of beneficial compounds like antcins and antrodin C, which have anti-cancer and anti-inflammatory properties. By analyzing gene expression patterns, they identified key genes and regulatory mechanisms that control the production of these medicinal compounds, suggesting ways to improve cultivation methods for better medicinal value.
Background
Taiwanofungus gaoligongensis is a newly identified fungal species with bioactive secondary metabolites including triterpenoids and antcins. Different cultivation methods can significantly affect the composition and yield of these bioactive compounds, but the underlying biosynthetic mechanisms remain poorly understood.
Objective
To investigate the effects of various cultivation methods on secondary metabolite production in T. gaoligongensis using multi-omics analysis, including transcriptomic and metabolomic approaches to elucidate biosynthetic pathways and transcriptional regulation of key bioactive compounds.
Results
NZJB and XZJB cultivation methods significantly enhanced terpenoid and triterpenoid production, with antcin C reaching 9.72-fold higher levels in XZJB and antcin I peaking at 12.83-fold higher in NZJB compared to rice medium. TgHMGR was identified as a key rate-limiting enzyme in terpenoid biosynthesis, with transcription factors TgHSF4, TgMYB6, TgZnF1, and others regulating target genes in the biosynthetic pathway.
Conclusion
Cultivation in C. kanehirae and C. camphora fungal cultivation bags significantly promotes accumulation of bioactive triterpenoid metabolites. The study provides insights into transcriptional regulation of antcin and antrodin C biosynthesis, suggesting strategies to enhance active compound production in artificially cultivated mycelia and improve medicinal value.
- Published in:Frontiers in Microbiology,
- Study Type:Experimental Research,
- Source: PMID: 40822405, doi: 10.3389/fmicb.2025.1620693