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Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation in Hypsizygus marmoreus

Summary

Scientists studied how beech mushrooms develop during a special low-temperature fruiting process that requires a long waiting period. They discovered that amino acids (particularly arginine) and citric acid accumulate during this process, and that adding these compounds externally can speed up mushroom production by 10 days and increase yield by 10-15%. This research could help mushroom farmers reduce costs and time in cultivation.

Background

Hypsizygus marmoreus (beech mushroom) undergoes a unique developmental process involving a month-long postripening stage followed by low-temperature fruiting. Understanding the molecular mechanisms underlying this low-temperature fruiting after long postripening (LFLP) is essential for optimizing mushroom cultivation and reducing production costs in the edible fungi industry.

Objective

To elucidate the molecular mechanisms of low-temperature fruiting after long postripening in H. marmoreus through integrative multi-omics analysis, including transcriptomics, amino acid metabolomics, and organic acid metabolomics, combined with genetic and physiological confirmation.

Results

Multi-omics analysis revealed that enhanced ribosomal pathway activity, accumulation of amino acids (particularly arginine), and increased citric acid levels are critical for LFLP. GCN2-mediated translation pathway was activated during low-temperature fruiting. Addition of citric acid and arginine shortened postripening by 10 days and increased fruiting body yield by 10.2-15.5% compared to controls.

Conclusion

The study reveals that GCN2-mediated protein translation is essential for LFLP in H. marmoreus, with synergistic accumulation of citric acid and arginine promoting amino acid biosynthesis and ATP production. The findings provide practical applications for mushroom cultivation optimization and potential breeding strategies for superior varieties with enhanced nutrient accumulation.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Research,
  • Source: PMID: 35887452, DOI: 10.3390/jof8070695
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