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Molecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods

Summary

This study developed and compared two molecular methods to accurately identify different yeast species that cause nail infections. Researchers tested 269 patient samples and found that both PCR-RFLP and PCR-FSP methods were effective, with PCR-FSP being better at identifying rare yeast species. These methods help doctors identify the exact type of yeast causing infection, which is important for selecting the right antifungal medication for treatment.

Background

Onychomycosis is a common fungal infection affecting nails, with increasing incidence of yeast-caused infections. Conventional diagnostic methods cannot reliably identify yeast isolates at the species level, making molecular methods essential for accurate identification.

Objective

This study aimed to identify yeast communities isolated from nail specimens using PCR-RFLP and PCR-FSP molecular methods to provide accurate species-level identification for appropriate treatment selection.

Results

Of 269 patients, 78 (28.99%) yeast strains were identified. Candida albicans was most common, followed by C. parapsilosis complex and C. tropicalis. Uncommon species including C. utilis, C. pararugosa, C. nivariensis, and Rhodotorula rubra were identified. PCR-FSP showed strong agreement with PCR-RFLP (κ=0.84).

Conclusion

Molecular diagnostic tools should complement conventional methods in clinical laboratories. PCR-FSP showed superior ability to identify rare yeasts, while PCR-RFLP effectively identified common species. Both methods have limitations in discriminating certain uncommon yeasts.
  • Published in:Current Medical Mycology,
  • Study Type:Experimental Study,
  • Source: PMID: 39744342, DOI: 10.22034/cmm.2024.345237.1539
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