Modified Recipe to Inhibit Fruiting Body Formation for Living Fungal Biomaterial Manufacture
- Author: mycolabadmin
- 2019-05-13
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Summary
This research demonstrates a new way to control mushroom growth in fungal materials by using specific chemical compounds. Instead of killing the fungal tissue with heat, which is the current practice, this method keeps the fungus alive but prevents it from forming mushrooms. This advancement is important for developing better sustainable materials from fungi.
Impacts on everyday life:
• Enables production of more durable and self-repairing fungal-based materials for packaging and construction
• Contributes to development of more sustainable alternatives to plastic and other synthetic materials
• Could lead to more efficient and cost-effective production of mushroom-based products
• Helps advance the field of sustainable, biodegradable materials for consumer goods
• May reduce manufacturing costs for fungal-based products, making them more accessible to consumers
Background
Living fungal mycelium-based materials have valuable applications due to their self-healing properties and ability to sense environmental changes. However, preventing fruiting body formation while keeping the mycelium alive has been a challenge in producing these materials. Current methods involve heat killing the mycelium, which eliminates many beneficial properties of living materials. A biochemical solution is needed to regulate fruiting body formation while maintaining living mycelium.
Objective
To develop a biochemical approach using glycogen synthase kinase-3 (GSK-3) inhibitors to regulate fruiting body formation in mushroom-forming fungi, providing an alternative to heat killing mycelium in the production of fungal biomaterials.
Results
Higher concentrations of GSK-3 inhibitors showed stronger inhibitory effects on fruiting body development. LiCl at 1.5-3 g/L promoted mycelial growth while preventing fruiting body formation. The stages before stage-1 primordium were found to be sensitive to LiCl treatment. Gene expression analysis showed reduced expression of GSK-3 and its target genes under LiCl treatment.
Conclusion
GSK-3 inhibitors like LiCl and CHIR99021 trihydrochloride can effectively inhibit fruiting body formation while maintaining living mycelium, providing an innovative strategy for easy, reliable, and low-cost maintenance of living fungal mycelium-based materials. This approach offers advantages over current heat-killing methods by preserving the beneficial properties of living materials.
- Published in:PLOS One,
- Study Type:Laboratory Research,
- Source: 10.1371/journal.pone.0209812