MYCOLab Ai Logo - gold and army green

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

Summary

This research examined how growing oyster mushrooms (P. columbinus) on different agricultural waste materials affects their health-promoting properties. The study found that the growing substrate significantly influences the mushrooms’ ability to fight microbes and act as antioxidants. This has important implications for both sustainable agriculture and human health. Impacts on everyday life: • Provides a way to convert agricultural waste into valuable nutritional supplements • Offers natural alternatives to synthetic antimicrobial compounds • Demonstrates sustainable methods for producing health-promoting foods • Suggests new ways to enhance the medicinal properties of edible mushrooms • Could lead to improved natural food preservatives

Background

The genus Pleurotus comprises a cosmopolitan group of mushrooms highly appreciated for their nutritional value and health-promoting benefits. These mushrooms are classified as white-rot fungi and represent one of the most widely cultivated edible mushroom groups worldwide. Despite many studies on Pleurotus species phytochemistry, there are very few reports specifically examining P. columbinus.

Objective

To investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation on the bioactive chemical profile of fruiting bodies and evaluate their potential as antioxidants and antimicrobials. Additionally, to conduct quantitative analysis of phenolic and flavonoid compounds potentially involved in the observed biological effects.

Results

The metabolomic analysis identified 97 significant metabolites. The fungi grown in substrate D showed higher levels of carbohydrates compared to the reference substrate A, while substrate B related to higher amounts of amino acids. Extract C was richest in phenolic compounds. All extracts displayed antimicrobial activity in concentrations ranging from 6.25 to 200 μg/mL, with particular effectiveness against E. coli. For antioxidant activity, extract B showed the strongest DPPH radical scavenging activity with EC50 of 2.25 μg/mL.

Conclusion

The findings support further investigation into how growth substrates influence P. columbinus antimicrobial and antioxidant properties. The extracts revealed valuable sources of primary and secondary metabolites, suggesting potential applications in food supplements with biological properties, particularly regarding antioxidant and antimicrobial effects. Future studies are needed to better understand the metabolic pathways and mechanisms of action underlying the observed effects.
  • Published in:Antibiotics (Basel),
  • Study Type:Experimental Laboratory Study,
  • Source: 10.3390/antibiotics10101245
Scroll to Top