Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Summary

This research examined how oyster mushrooms respond metabolically to heat stress. The study revealed that when exposed to high temperatures, the mushrooms alter their internal chemistry to try to protect themselves and survive. This has important implications for mushroom cultivation and understanding stress responses in fungi. Impacts on everyday life: – Helps improve commercial mushroom cultivation techniques in warm climates – Provides insights for developing more heat-resistant mushroom strains – Advances understanding of how fungi adapt to environmental stress – Could lead to better storage and transport conditions for mushrooms – May help predict and prevent mushroom crop losses due to heat

Background

Heat stress seriously threatens the growth of Pleurotus ostreatus (oyster mushroom). While various studies have examined P. ostreatus heat resistance, the metabolic response mechanisms remain unclear.

Objective

To evaluate the metabolome and determine the response of P. ostreatus mycelia to heat stress at different time points (6, 12, 24, 48 hours) and analyze the dynamic metabolite changes.

Results

Over 70 differential metabolites were detected and enriched in metabolic pathways. Heat stress enhanced degradation of unsaturated fatty acids and nucleotides, increased amino acid and vitamin content, and accelerated glycolysis and the tricarboxylic acid cycle. Amino acids showed continuous changes, nucleotides changed notably at 12 and 48 hours, and lipids exhibited increasing trends with prolonged heat stress. Heat treatment induced production of stress-resistant substances like salicylic acid that are typically found in plants.

Conclusion

This study provides the first comprehensive analysis of metabolite changes in P. ostreatus mycelia during 48 hours of heat stress. The identified metabolic pathways and substances that changed under heat stress provide valuable insights for future research on heat resistance mechanisms in P. ostreatus and other edible fungi.
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