Metabarcoding and Metabolome Analyses Reveal Mechanisms of Leymus chinensis Growth Promotion by Fairy Ring of Leucocalocybe mongolica

Summary

This research investigated how fairy rings – circular patterns of mushroom growth in grasslands – can promote plant growth naturally without fertilizers. The study found that these fairy rings change the beneficial microbes living inside plants and alter plant metabolism in ways that enhance growth and stress resistance. This has important real-world implications: • Could reduce chemical fertilizer use in agriculture • Provides a natural way to improve grass growth for livestock grazing • Demonstrates sustainable methods for improving crop yields • Helps understand how fungi and bacteria work together to benefit plants • Could lead to development of new bio-based fertilizers

Background

Fairy rings are unique ecological phenomena caused by fungal mycelium growth in soil that can promote plant growth without fertilization. Understanding their mechanisms could help develop sustainable agricultural practices. Previous research investigated soil factors, but plant-specific aspects remained unexplored.

Objective

To investigate the endophyte diversity and metabolome of Leymus chinensis (LC) in a Leucocalocybe mongolica (LM) fairy ring ecosystem by analyzing leaf and root samples from the DARK (FR) and OUT (CK) zones.

Results

The fairy rings significantly improved fungal diversity in roots and leaves and bacterial diversity in leaves. Ralstonia was the dominant bacteria in LC leaves, while Marasmius was highly abundant in FR zone roots. Metabolome analysis identified 1011 metabolites and seven significantly regulated pathways. The fairy ring ecosystem significantly downregulated flavonoid metabolism in leaves and roots while upregulating salicylic acid and jasmonic acid in leaves.

Conclusion

The fairy ring ecosystem regulates endophytic diversity and metabolic processes to promote LC growth. The study identified key factors including Marasmius enrichment, Ralstonia abundance, and flavonoid downregulation as important mechanisms. These findings provide a theoretical foundation for developing fairy ring ecosystems in agricultural systems.
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