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Low temperature, mechanical wound, and exogenous salicylic acid (SA) can stimulate the SA signaling molecule as well as its downstream pathway and the formation of fruiting bodies in Flammulina filiformis

Summary

Researchers studied how cooling, physical damage, and a plant hormone called salicylic acid can trigger fruiting body formation in an edible mushroom called Flammulina filiformis. They discovered that these treatments activate specific genes in the mushroom that control fruit production. This research helps explain why mushroom farmers use these methods and could improve mushroom cultivation efficiency.

Background

Flammulina filiformis is an industrially important edible mushroom. Low temperature and mechanical wounding are empirically used in production to stimulate fruiting body formation, but the underlying mechanisms involving endogenous hormones and signaling pathways remain poorly understood.

Objective

This study investigated how low temperature, mechanical wound, and exogenous salicylic acid treatments affect fruiting body formation and activate the SA signaling pathway and its downstream genes in F. filiformis.

Results

All four treatments accelerated primordia formation (appearing by day 5-6 versus day 12 in control) and significantly increased endogenous SA content (1.3-2.6 fold). Eleven SA signaling genes were identified: 4 NPR genes, 5 TGA genes, and 2 PR genes, with FfNpr3 showing significant upregulation under all treatments. FfTga and FfPr genes also showed 1.6-8.5 fold significant upregulation.

Conclusion

A correlation exists between low temperature/mechanical wound stress, SA signaling activation, and fruiting body formation in F. filiformis. The SA signaling pathway, particularly FfNpr3 and downstream TGA/PR genes, plays a central role in fruiting body formation in this fungus.
  • Published in:Frontiers in Microbiology,
  • Study Type:Experimental Study,
  • Source: 10.3389/fmicb.2023.1197498; PMID: 37675426
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