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Kojic Acid-Mediated Damage Responses Induce Mycelial Regeneration in the Basidiomycete Hypsizygus marmoreus

Summary

This research explores how kojic acid helps mushrooms recover from mechanical damage and produce more fruiting bodies. The findings have important implications for mushroom cultivation and understanding cellular repair mechanisms. Impact on everyday life: – Improved methods for commercial mushroom production – Better understanding of how organisms heal from physical damage – Potential applications in natural antioxidant treatments – Insights into cellular stress response mechanisms – Applications for enhancing crop yields through stress management

Background

Mechanical damage can induce fruiting body production in fungi, but the molecular mechanisms involved in this response are not well understood. In Hypsizygus marmoreus production, mechanical scraping of mature mycelia is required to induce primordium initiation, though excessive injury can delay fruiting body development.

Objective

To investigate how kojic acid (KA) enhances injured mycelial regeneration and increases fruiting body production in H. marmoreus, and to understand the molecular mechanisms and signaling pathways involved in this response.

Results

KA treatment enhanced mycelial regeneration and increased fruiting body production. It reduced harmful ROS levels while increasing antioxidant enzyme activities (CAT and SOD) and antioxidant contents (GSH and AsA). KA increased extracellular ATP levels and modulated ROS signaling by decreasing NADPH oxidase gene expression. The treatment upregulated MAPK, calcium and oxylipin signaling pathways. Laccase and cellulase activities were stimulated by KA at different developmental stages.

Conclusion

Kojic acid promotes mycelial regeneration in H. marmoreus by activating stress signals and enhancing oxidative damage resistance through the antioxidant system. It regulates multiple signaling pathways involved in wound healing, mycelial regeneration and reproductive structure formation. The findings demonstrate KA’s role in modulating both damage response and fruiting body development in this commercially important mushroom species.
  • Published in:PLoS One,
  • Study Type:Laboratory Research,
  • Source: 10.1371/journal.pone.0187351
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