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iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

Summary

This study examined how oyster mushrooms respond to high temperatures using advanced protein analysis techniques. Researchers found that when mushroom mycelium was exposed to 40°C heat, it damaged cell membranes and changed the levels of hundreds of proteins. However, when the temperature returned to normal, the mushrooms could repair the damage and recover. Key proteins including heat shock proteins and stress-response enzymes played important roles in protecting the mushroom cells and helping them survive heat stress.

Background

High temperature is a critical limiting factor for mycelium growth and development in Pleurotus ostreatus (oyster mushroom), the third largest edible fungus produced in China. Heat stress causes irreversible damage to growth, impairs fruiting, and affects mushroom quality. Understanding the thermotolerance mechanisms at the molecular level is essential for improving cultivation in agricultural facilities.

Objective

To investigate the proteomic changes in P. ostreatus mycelium in response to heat stress at 40°C for 48 hours followed by recovery at 25°C for 3 days using iTRAQ-based quantitative proteomics technology. The study aimed to identify key proteins and molecular mechanisms involved in heat stress response and recovery.

Results

A total of 204 differentially expressed proteins (DEPs) were identified, with 47 proteins responding to both stress and recovery conditions, 84 responding only to heat stress, and 73 to recovery only. Key proteins involved in MAPK signaling, antioxidant defense, heat shock proteins (HSP60, HSP90, HSP104), and glycolysis were upregulated. TBARS concentration significantly increased during heat stress but returned to control levels during recovery, indicating functional repair of heat-induced cell membrane damage.

Conclusion

The study demonstrates that effective regulatory protein expression related to MAPK pathway, antioxidant enzymes, heat shock proteins, and glycolysis play crucial roles in enhancing P. ostreatus adaptation to and recovery from heat stress. The findings provide insights into the thermotolerance mechanisms for basidiomycetes and suggest strategies for improving heat tolerance in edible mushroom cultivation.
  • Published in:Frontiers in Microbiology,
  • Study Type:Experimental Study,
  • Source: PMID: 30356767, DOI: 10.3389/fmicb.2018.02368
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