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Isolation and Purification of Two Isoflavones from Hericium erinaceum Mycelium by High-Speed Counter-Current Chromatography

Summary

This research successfully isolated and identified two beneficial compounds (genistein and daidzein) from lion’s mane mushroom for the first time using an advanced separation technique. These compounds are known to have various health benefits including antioxidant and anti-inflammatory properties. Impacts on everyday life: • Provides scientific evidence for the traditional use of lion’s mane mushroom in health supplements • Helps validate the potential health benefits of consuming lion’s mane mushroom products • Enables better quality control and standardization of lion’s mane supplements • Could lead to development of new natural health products • Advances our understanding of the active compounds in medicinal mushrooms

Background

Hericium erinaceum (lion’s mane mushroom) is a traditional edible mushroom used in East Asian medicine with various bioactivities including antioxidant, immune regulatory, anti-aging, antimicrobial, anti-inflammatory and anti-cancer properties. While flavonoids are known to be present in H. erinaceum and contribute to its antioxidant effects, specific flavonoid compounds had not previously been identified and isolated from this species.

Objective

To develop and optimize a high-speed counter-current chromatography (HSCCC) method for separating and purifying isoflavones from H. erinaceum mycelium for the first time, and to identify the chemical structures of the isolated compounds.

Results

Two isoflavones were successfully isolated and identified: 23 mg of genistein (4′,5,7-trihydroxyisoflavone) with 95.7% purity and 18 mg of daidzein (4′,7-dihydroxyisoflavone) with 97.3% purity were obtained from 150 mg of ethanolic extract of H. erinaceum mycelium.

Conclusion

This study demonstrated that HSCCC is an effective method for separating and purifying isoflavones from H. erinaceum mycelium. This represents the first isolation and identification of genistein and daidzein from this species, establishing HSCCC as a powerful tool for quick and efficient separation of bioactive compounds from natural products.
  • Published in:Molecules,
  • Study Type:Laboratory Research,
  • Source: 10.3390/molecules23030560
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