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Isolation and Identification of Bioactive Compounds from Antrodia camphorata Against ESKAPE Pathogens

Summary

This research discovered a new natural compound from the medicinal mushroom Antrodia camphorata that could help fight antibiotic-resistant bacteria. The compound, called MBBD, was particularly effective against dangerous hospital-acquired infections while showing no toxicity to normal human cells. Impact on everyday life: – Could lead to new antibiotics to treat resistant infections – Offers a potential natural alternative to synthetic antibiotics – May help reduce the threat of untreatable bacterial infections – Could lower healthcare costs associated with treating resistant infections – Demonstrates the ongoing value of natural products in modern medicine

Background

Antimicrobial resistance is a major global health threat, with drug-resistant bacteria causing 1.27 million direct deaths worldwide in 2019. ESKAPE pathogens (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli) are common opportunistic pathogens causing nosocomial infections with high morbidity and mortality. New antibacterial drugs are urgently needed to tackle bacterial resistance.

Objective

To investigate the antimicrobial activity of Antrodia camphorata fermentation broth and isolate, purify, and identify the antimicrobial compounds present using an antimicrobial activity tracking method.

Results

A compound identified as 5-methyl-benzo[1,3]-dioxole-4,7-diol (MBBD) was isolated and showed broad-spectrum antibacterial activity. The MIC range for drug-resistant pathogenic bacteria was 64-256 μg/mL, with lowest MIC against A. baumannii (64 μg/mL). The MIC range against 10 foodborne pathogens was 12.5-100 μg/mL. MBBD showed no cytotoxicity against human normal lung epithelial cells.

Conclusion

MBBD isolated from A. camphorata exhibits broad-spectrum antibacterial activity, particularly demonstrating excellent inhibitory effects against A. baumannii. The compound shows promise as a candidate for new antimicrobial drugs, especially given its lack of toxicity to normal human cells.
  • Published in:PLoS One,
  • Study Type:Laboratory Research,
  • Source: 10.1371/journal.pone.0293361
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